Viral Genomes and Antigen Detection of Hepatitis B and C Viruses in Involved Lymph Nodes of Egyptian Non-Hodgkin's Lymphoma Patients

¹Ghada Mohamed El-sayed, ²Waleed Seif El-din Mohamed, ³Mohamed Akram Nouh, ⁴Manar Mohamed Moneer and ⁵Hadir Ahmed EL-Mahallawy

Departments of ¹Clinical Pathology, ²Virology & Immunology, ³Surgical Pathology, ⁴Epidemiology and ⁵Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt.

Several studies have suggested an association between Hepatitis C and B viruses (HCV and HBV) and non-Hodgkin's lymphoma (NHL). In the present study we have searched for viral genomes and antigens in the malignant lymphoma tissues as well as their seroprevalence. Antibodies against Hepatitis C as well as HCV RNA and hepatitis B surface antigen (HBsAg) were determined for 29 newly diagnosed non-Hodgkin's lymphoma patients using an enzyme linked immunosorbent assay (ELISA), as well as RT-PCR and compared with 36 apparently healthy individuals as a control group for viral markers. Immunohistochemical staining (IHC) was performed on paraffin embedded tissues for the NS3 of HCV and for HBsAg of HBV using the immunoperoxidase technique. Paraffin embedded lymph nodes (LN) were studied for the presence of viral sequences. Ten non-metastatic lymph nodes (LN) from cancer cases other than NHL were used as a control for IHC and molecular studies. HCV was significantly more encountered in patients with NHL when compared to controls for both antibodies (27.6% versus 8.3% of serum controls; p=0.04), and antigens studied by IHC in the involved LN (41% versus 10% of tissue controls; p=0.06). Although HBsAg positivity was not different in NHL patients when compared to controls (6.9% and 2.7%); yet it was significantly more encountered in LN of NHL patients (p=0.04). HBV-DNA was detected in 27.5% of patient's samples and none of the controls. In conclusion, overall our findings confirm the presence of HBV and HCV antigens and viral sequences in the involved LNs of NHL patients, except for HCV RNA which perhaps necessitates fresh and not paraffin embedded tissues. These results strengthen the assumption that these viruses may be involved in the development of NHL.