1El-Enbaawy, M.I., 2Saad, M.M. and 3Selim,
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, 2Center for Biotechnology Services and Researches, Faculty of Veterinary Medicine, Cairo University, 3Department of Reproduction Diseases, Animal Reproduction Research Institute, Al-Haram, Giza, Egypt.
Humoral and cellular immune responses were both found to be
operative in five groups of Balb/c mice following two subcutaneous inoculations
with different antigens of C. pseudotuberculosis. These antigens
included toxoid, bacterin, bacterin-toxoid with and without oil adjuvant in
addition to the live cell of C. pseudotuberculosis. The responses were
assessed, twenty days after the 2nd immunization. Serum antibody levels were
determined in an enzyme linked immunosorbent assay (ELISA). Cellular
immune responses to C. pseudotuberculosis antigens were measured by
detection of gamma interferon (IFN- gamma) in spleen cell culture media of the
immunized mice, using commercial mice enzyme immuno-assay kit. All mice were
challenged 2 weeks after the last dose of immunization with live C.
pseudotuberclosis (2x105 CFU /mouse). Protection levels were
observed with different degrees between the immunized mice groups.